"Prutiìna fluoriscenti virdi" : Diffirenzi ntrê virsioni

[[Image:GFP structure.png|thumb|200px|Lu tiaggramma a nastru da prutiina fluoriscenti virdi (GFP, l'acronimu angrisi)]]

[[Image:Aequorea victoria.jpg|thumb|200px|''Aequorea victoria'']]

Nta lu 2008 [[Martin Chalfie]], [[Osamu Shimomura]] e [[Roger Y. Tsien]] ci appiru lu [[Premiu Nobel nta chimica]] pâ scuperta e lu sviluppu dâ prutiìna fluoriscenti virdi.<br>

 

== Storia ==

 

Li sordi ppi stu pruggettu finièrru e allura Prasher mannau lu [[cDNA]] samples to several labs. The lab of [[Martin Chalfie]] expressed the coding sequence of wtGFP, with the first few amino acids deleted, in heterologous cells of ''[[E. coli]]'' and ''[[Caenorhabditis elegans|C. elegans]]'', publishing the results in ''Science'' in 1994.<ref name=Chalfie_1994>{{cite journal |author=Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher D |title=Green fluorescent protein as a marker for gene expression |journal=Science |volume=263 |issue=5148 |pages=802–5 |year=1994 |pmid=8303295 |doi=10.1126/science.8303295}}</ref> Frederick Tsuji's lab independently reported the expression of the recombinant protein one month later.<ref name=Inouye_1994>{{cite journal |author=Inouye S, Tsuji F |title=Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein |journal=FEBS Lett |volume=341 |issue=2-3 |pages=277–80 |year=1994 |pmid=8137953 |doi=10.1016/0014-5793(94)80472-9}}</ref> Remarkably, the GFP molecule folded and was fluorescent at room temperature, without the need for exogenous cofactors specific to the jellyfish. Although this near-wtGFP was fluorescent, it had several drawbacks, including dual peaked excitation spectra, pH sensitivity, chloride sensitivity, poor fluorescence quantum yield, poor photostability and poor folding at 37°C.